It sounds as though queenie has found herself a comfy place to sleep and doesn't want to be ousted, but she has to learn to sleep in the coop with everyone else. If you keep putting her in there she will eventually get the message. Can you move the bike out of her way so it's not an option at bedtime?
As for cockerels, it's 50/50 what you might get. I have two cockerels (did have three until recently) and all of them are gentle with me and their girls, all but one I have raised from a chick so they know me well. You need to handle them alot when young, so they know you. However, you can always get a rogue that will become agressive even though they know you and this can cause injury problems - very serious ones if you have young children. On some cocks the spurs can be over 2 inches long! I have scars on each leg from a cockerel attack from when I was a child and I wouldn't wish that experience on anyone.
As to changing the personality of the flock, I can't honestly say that anything bad has happened with mine. If anything I think it has improved mine as it's lovely to see the cockerel calling his girls over for tasty titbits he's found or calling the alarm if there is a heron or bird of prey about. But there's also the downside of the noise - cockerels start very early in the summer (about 4:30am!) and thankfully, I have very understanding neighbours but others on this site have not been so lucky, so that may be something you will need to consider also.